| 质粒名称: | pKDEL-mScarlet3-H (aka mYongHong) |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000759 |
| 产品用途: | Expresses mScarlet3-H localized in ER |
| 存储实验室: | Zhifei Fu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | mScarlet3-H |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A highly stable monomeric red fluorescent protein for advanced microscopy
Xiong H., Chang Q., Ding J., Wang S., Zhang W., Li Y., Wu Y., Lin P., Yang C., Liu M., Fang G., Yang Y., Xie J., Qi D., Jiang T., Fu W., Hu F., Chen Y., Yue R., Li Y., Cui Y., Li M., Fan S., Yang Y., Xu Y., Li D., Zhang F., Zhao H., Wu C., Zheng Q., Piatkevich KD., Fu Z.
Nat. Methods 2025 1–11 (2025)
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pKDEL-mScarlet3-H (aka mYongHong)
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pKDEL-mScarlet3-H (aka mYongHong)载体质粒基本信息
pKDEL-mScarlet3-H (aka mYongHong)质粒图谱载体图谱和pKDEL-mScarlet3-H (aka mYongHong)载体序列质粒序列多克隆位点信息
载体图谱.png)
pKDEL-mScarlet3-H (aka mYongHong)质粒载体简介
pKDEL-mScarlet3-H (aka mYongHong)质粒序列载体序列
LOCUS pKDEL-mScarlet3-H (aka mYongHong) 4743 bp DNA circular SYN 30-DEC-2024
DEFINITION TSN20241013-0571-00010_759_20241014-BAN4_B04_B04_i20.1.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4743)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 23, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4743
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 66..170
/gene="bla"
/label=AmpR promoter
promoter 172..529
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 380..515
/label=SV40 ori
/note="SV40 origin of replication"
CDS 564..1358
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 1590..1637
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 1966..2554
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
enhancer 2729..3032
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3033..3236
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
regulatory 3325..3334
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 3331..4017
/codon_start=1
/label=mScarlet3-H
/translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKH
ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
TGGSGGS"
polyA_signal 4199..4320
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(join(4327..4743,1..39))
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
61 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
121 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg
181 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
241 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
301 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
361 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
421 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
481 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag
541 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
601 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
661 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
721 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
781 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
841 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
901 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
961 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
1021 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
1081 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
1141 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
1201 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
1261 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
1321 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
1381 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
1441 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
1501 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
1561 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
1621 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
1681 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
1741 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
1801 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
1861 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
1921 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
1981 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
2041 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
2101 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtatc
2161 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
2221 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
2281 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
2341 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
2401 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
2461 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
2521 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
2581 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
2641 tgtggataac cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat
2701 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
2761 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
2821 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
2881 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
2941 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
3001 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
3061 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
3121 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
3181 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt
3241 tagtgaaccg tcagatccgc tagcatgctg ctatccgtgc cgttgctgct cggcctcctc
3301 ggcctggccg tcgccgcacc ggtcgccacc atggatagca ccgaggcagt gatcaaggag
3361 ttcatgcggt tcaaggtgca catggagggc tccatgaacg gccacgagtt cgagatcgag
3421 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgag ggtgaccaag
3481 ggtggccccc tgcccttctc ctgggacatc ctgtcccctc agttcatgta cggctccagg
3541 gccttcacga agcaccccgc cgacatcccc gactactgga agcagtcctt ccccgagggc
3601 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg ccgtgtccgt ggcccaggac
3661 acctccctgg aggacggcac cctgatctac aaggtgaagc tccgcggcac caacttccct
3721 cctgacggcc ccgtaatgca gaagaagaca atgggctggg aagcatccac cgagcggttg
3781 taccccgagg acgtcgtgct gaagggcgac attaagcatg ccctgcgcct gaaggacggc
3841 ggccggtacc tggcggactt caagaccacc tacagggcca agaagcccgt gcagatgccc
3901 ggcgccttca acatcgaccg caagttggac atcacatccc acaacgagga ctacaccgtg
3961 gtggaacagt acgaacgctc cgtggcccgc cactccaccg gcggctccgg tggctcctcc
4021 ggaaaggacg agctgtaaga attctgcagt cgacggtacc gcgggcccgg gatccaccgg
4081 atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
4141 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
4201 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
4261 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
4321 aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
4381 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
4441 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
4501 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
4561 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
4621 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
4681 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
4741 cca
//
pKDEL-mScarlet3-H (aka mYongHong)载体图谱质粒图谱pdf版和pKDEL-mScarlet3-H (aka mYongHong)载体序列质粒序列等相关资料下载
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